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il-33 concentrations elisa kits  (R&D Systems)


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    R&D Systems il-33 concentrations elisa kits
    <t>IL-33</t> increased in the lung and BALF 1 h after LPS-induced lung injury. WT mice were administered an intratracheal injection of LPS, and then the lung tissue and BALF were collected for 1, 3, and 24 h after ARDS induction. A, The mRNA expression level <t>of</t> <t>IL-33</t> was detected by quantitative polymerase chain reaction (n = 5). In the BALF (B) and lung (C), IL-33 protein concentrations were determined via ELISA (n = 5–8) and western blotting (E–D) (n = 5–8). Total protein in the lung (C) was measured by BCA. F, An anti-IL-33 antibody was used for the immunohistochemistry analysis of lung tissue (n = 5). IL-33 − / − mice were used as the negative controls. Arrowheads point to the nuclear translocation of IL-33. Scale bar = 50 μm. G, Lung protein concentrations in lung tissue were determined via western blotting (n = 3), and (H) was the typical picture. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; and KO, knockout; WT, wild-type.
    Il 33 Concentrations Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il-33+concentrations+elisa+kits/enzyme+linked+immunosorbent+assay++elisa++kits/pmc10227934-49-5-18
    Average 90 stars, based on 1 article reviews
    il-33 concentrations elisa kits - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS"

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS

    Journal: Shock (Augusta, Ga.)

    doi: 10.1097/SHK.0000000000002114

    IL-33 increased in the lung and BALF 1 h after LPS-induced lung injury. WT mice were administered an intratracheal injection of LPS, and then the lung tissue and BALF were collected for 1, 3, and 24 h after ARDS induction. A, The mRNA expression level of IL-33 was detected by quantitative polymerase chain reaction (n = 5). In the BALF (B) and lung (C), IL-33 protein concentrations were determined via ELISA (n = 5–8) and western blotting (E–D) (n = 5–8). Total protein in the lung (C) was measured by BCA. F, An anti-IL-33 antibody was used for the immunohistochemistry analysis of lung tissue (n = 5). IL-33 − / − mice were used as the negative controls. Arrowheads point to the nuclear translocation of IL-33. Scale bar = 50 μm. G, Lung protein concentrations in lung tissue were determined via western blotting (n = 3), and (H) was the typical picture. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; and KO, knockout; WT, wild-type.
    Figure Legend Snippet: IL-33 increased in the lung and BALF 1 h after LPS-induced lung injury. WT mice were administered an intratracheal injection of LPS, and then the lung tissue and BALF were collected for 1, 3, and 24 h after ARDS induction. A, The mRNA expression level of IL-33 was detected by quantitative polymerase chain reaction (n = 5). In the BALF (B) and lung (C), IL-33 protein concentrations were determined via ELISA (n = 5–8) and western blotting (E–D) (n = 5–8). Total protein in the lung (C) was measured by BCA. F, An anti-IL-33 antibody was used for the immunohistochemistry analysis of lung tissue (n = 5). IL-33 − / − mice were used as the negative controls. Arrowheads point to the nuclear translocation of IL-33. Scale bar = 50 μm. G, Lung protein concentrations in lung tissue were determined via western blotting (n = 3), and (H) was the typical picture. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; and KO, knockout; WT, wild-type.

    Techniques Used: Injection, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Translocation Assay, Knock-Out

    The IL-33/ST2 axis promotes lung damage in LPS-induced ARDS. BALF and lung tissue were harvested after LPS administration for 24 h. A, The lung D/W ratio was assessed to evaluate lung edema (n = 5). B, Protein concentration and neutrophil count (C) in the BALF were analyzed with a BCA kit and flow cytometry to detect epithelial permeability (n = 5). D and E, Histopathological images of the lung tissues are based on one representative image among five (HE staining), and the scale bar = 50 μm. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; ctr, control; D/W, dry/wet; HE, hematoxylin-eosin; KO, knockout; WT, wild-type.
    Figure Legend Snippet: The IL-33/ST2 axis promotes lung damage in LPS-induced ARDS. BALF and lung tissue were harvested after LPS administration for 24 h. A, The lung D/W ratio was assessed to evaluate lung edema (n = 5). B, Protein concentration and neutrophil count (C) in the BALF were analyzed with a BCA kit and flow cytometry to detect epithelial permeability (n = 5). D and E, Histopathological images of the lung tissues are based on one representative image among five (HE staining), and the scale bar = 50 μm. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; ctr, control; D/W, dry/wet; HE, hematoxylin-eosin; KO, knockout; WT, wild-type.

    Techniques Used: Protein Concentration, Flow Cytometry, Permeability, Staining, Knock-Out

    IL-33 and ST2 deletion leads to reduced levels of circulating and BALF proinflammatory cytokines. The concentrations of cytokines in the serum and BALF were measured 24 h after ARDS induction and by CBA. The IL-6, MCP-1, and TNF levels in the plasma (A) and BALF (B) of WT mice, IL-33 − / − mice, and ST2 − / − mice were measured to evaluate the systemic inflammatory response (n = 5). The data are shown as the mean + SD * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; CBA, bead-based multiplex immunoassay; ctr, control; KO, knockout; WT, wild-type.
    Figure Legend Snippet: IL-33 and ST2 deletion leads to reduced levels of circulating and BALF proinflammatory cytokines. The concentrations of cytokines in the serum and BALF were measured 24 h after ARDS induction and by CBA. The IL-6, MCP-1, and TNF levels in the plasma (A) and BALF (B) of WT mice, IL-33 − / − mice, and ST2 − / − mice were measured to evaluate the systemic inflammatory response (n = 5). The data are shown as the mean + SD * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; CBA, bead-based multiplex immunoassay; ctr, control; KO, knockout; WT, wild-type.

    Techniques Used: Multiplex Assay, Knock-Out

    Mice lacking IL-33 and ST showed reduced ability to recruit and activate iNKT cells after ARDS induction. WT, IL-33 − / − , and ST2 − / − mice were subjected to a sham treatment or intratracheal LPS injection. After 24 h, the lung and spleen were obtained to prepare single-cell suspensions, and the cells were stained with anti-CD45, anti-CD3, anti-CD1d-tetramer, anti-NK1.1, anti-CD4, anti-CD8, and anti-CD69 antibodies. A and B, Representative dot plots. iNKT cells were identified as CD45 + CD3 + CD1d + tetramers (n = 5). C, The data are presented as the ratio of iNKT to CD3 + T cells and the CD69 geometric mean expression on iNKT cells in the lung and spleen (n = 3–5). D and E, Representative dot plots. NK cells were identified as CD45 + CD3 − NK1.1 + cells. F, The data are presented as the ratio of NK cells to CD3 − T cells and CD69 geometric mean expression on NK cells in the lung and spleen (n = 3–5). Three independent experiments were conducted to obtain the results shown. The data are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; Ctr, control; KO, knockout; WT, wild-type.
    Figure Legend Snippet: Mice lacking IL-33 and ST showed reduced ability to recruit and activate iNKT cells after ARDS induction. WT, IL-33 − / − , and ST2 − / − mice were subjected to a sham treatment or intratracheal LPS injection. After 24 h, the lung and spleen were obtained to prepare single-cell suspensions, and the cells were stained with anti-CD45, anti-CD3, anti-CD1d-tetramer, anti-NK1.1, anti-CD4, anti-CD8, and anti-CD69 antibodies. A and B, Representative dot plots. iNKT cells were identified as CD45 + CD3 + CD1d + tetramers (n = 5). C, The data are presented as the ratio of iNKT to CD3 + T cells and the CD69 geometric mean expression on iNKT cells in the lung and spleen (n = 3–5). D and E, Representative dot plots. NK cells were identified as CD45 + CD3 − NK1.1 + cells. F, The data are presented as the ratio of NK cells to CD3 − T cells and CD69 geometric mean expression on NK cells in the lung and spleen (n = 3–5). Three independent experiments were conducted to obtain the results shown. The data are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; Ctr, control; KO, knockout; WT, wild-type.

    Techniques Used: Injection, Staining, Expressing, Knock-Out

    The ratio of CD4 + T cells and CD8 + T cells to CD3 + T cells was not different after ARDS induction in IL-33 − / − and ST − / − mice. The lung and spleen were obtained to prepare single-cell suspensions, and the cells stained with anti-CD45, anti-CD3, anti-CD1d-tetramer, anti-NK1.1, anti-CD4, anti-CD8, and anti-CD69 antibodies 24 h after LPS-induced ARDS. A, The data are presented as the ratio of CD4 + T cells to CD3 + T cells and the CD69 geometric mean in CD4 + T cells in the lung and spleen (n = 5). B, The data are presented as the ratio of CD8+ T cells to CD3 + T cells and the CD69 geometric mean expression on CD8 + T cells in the lung and spleen (n = 5). The results shown are based on one of three independent experiments. The data are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; ctr, control; KO, knockout; WT, wild-type.
    Figure Legend Snippet: The ratio of CD4 + T cells and CD8 + T cells to CD3 + T cells was not different after ARDS induction in IL-33 − / − and ST − / − mice. The lung and spleen were obtained to prepare single-cell suspensions, and the cells stained with anti-CD45, anti-CD3, anti-CD1d-tetramer, anti-NK1.1, anti-CD4, anti-CD8, and anti-CD69 antibodies 24 h after LPS-induced ARDS. A, The data are presented as the ratio of CD4 + T cells to CD3 + T cells and the CD69 geometric mean in CD4 + T cells in the lung and spleen (n = 5). B, The data are presented as the ratio of CD8+ T cells to CD3 + T cells and the CD69 geometric mean expression on CD8 + T cells in the lung and spleen (n = 5). The results shown are based on one of three independent experiments. The data are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; ctr, control; KO, knockout; WT, wild-type.

    Techniques Used: Staining, Expressing, Knock-Out

    IL-33 contributes to an uncontrolled inflammatory response in ARDS via activation of NKT cells. WT and Vα14Τg mice were pretreated with an anti-ST2 antibody 1 h before LPS administration and sacrificed 24 h after ARDS induction. BALF and lung tissue were harvested. A, The D/W ratio was calculated to evaluate lung edema (n = 5). B, Protein concentration and neutrophil count (C) in the BALF were measured with a BCA kit and by flow cytometry to evaluate epithelial permeability (n = 5). D and E, One of five representative histopathological images showing the lung tissues (HE staining). The lung injury score was determined by two blinded pathologists. Scale bar = 50 μm. F, The levels of IL-6, MCP-1, and TNF in the plasma and BALF were tested by CBA. (n = 5, * P < 0.05). The data are shown as the mean ± SD and are representative of three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; ctr, control; D/W, dry/wet; KO, knockout; WT, wild-type.
    Figure Legend Snippet: IL-33 contributes to an uncontrolled inflammatory response in ARDS via activation of NKT cells. WT and Vα14Τg mice were pretreated with an anti-ST2 antibody 1 h before LPS administration and sacrificed 24 h after ARDS induction. BALF and lung tissue were harvested. A, The D/W ratio was calculated to evaluate lung edema (n = 5). B, Protein concentration and neutrophil count (C) in the BALF were measured with a BCA kit and by flow cytometry to evaluate epithelial permeability (n = 5). D and E, One of five representative histopathological images showing the lung tissues (HE staining). The lung injury score was determined by two blinded pathologists. Scale bar = 50 μm. F, The levels of IL-6, MCP-1, and TNF in the plasma and BALF were tested by CBA. (n = 5, * P < 0.05). The data are shown as the mean ± SD and are representative of three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; ctr, control; D/W, dry/wet; KO, knockout; WT, wild-type.

    Techniques Used: Activation Assay, Protein Concentration, Flow Cytometry, Permeability, Staining, Knock-Out

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Injection:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Expressing:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Real-time Polymerase Chain Reaction:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Western Blot:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Immunohistochemistry:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Translocation Assay:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Knock-Out:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Protein Concentration:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Flow Cytometry:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Permeability:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Staining:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Multiplex Assay:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Activation Assay:

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS
    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).



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    il-33 concentrations elisa kits  (R&D Systems)
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    R&D Systems il-33 concentrations elisa kits
    <t>IL-33</t> increased in the lung and BALF 1 h after LPS-induced lung injury. WT mice were administered an intratracheal injection of LPS, and then the lung tissue and BALF were collected for 1, 3, and 24 h after ARDS induction. A, The mRNA expression level <t>of</t> <t>IL-33</t> was detected by quantitative polymerase chain reaction (n = 5). In the BALF (B) and lung (C), IL-33 protein concentrations were determined via ELISA (n = 5–8) and western blotting (E–D) (n = 5–8). Total protein in the lung (C) was measured by BCA. F, An anti-IL-33 antibody was used for the immunohistochemistry analysis of lung tissue (n = 5). IL-33 − / − mice were used as the negative controls. Arrowheads point to the nuclear translocation of IL-33. Scale bar = 50 μm. G, Lung protein concentrations in lung tissue were determined via western blotting (n = 3), and (H) was the typical picture. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; and KO, knockout; WT, wild-type.
    Il 33 Concentrations Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il-33+concentrations+elisa+kits/enzyme+linked+immunosorbent+assay++elisa++kits/pmc10227934-49-5-18
    Average 90 stars, based on 1 article reviews
    il-33 concentrations elisa kits - by Bioz Stars, 2026-10
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    human il 33 concentration  (Danaher Inc)
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    Danaher Inc human il 33 concentration
    <t>IL-33</t> increased in the lung and BALF 1 h after LPS-induced lung injury. WT mice were administered an intratracheal injection of LPS, and then the lung tissue and BALF were collected for 1, 3, and 24 h after ARDS induction. A, The mRNA expression level <t>of</t> <t>IL-33</t> was detected by quantitative polymerase chain reaction (n = 5). In the BALF (B) and lung (C), IL-33 protein concentrations were determined via ELISA (n = 5–8) and western blotting (E–D) (n = 5–8). Total protein in the lung (C) was measured by BCA. F, An anti-IL-33 antibody was used for the immunohistochemistry analysis of lung tissue (n = 5). IL-33 − / − mice were used as the negative controls. Arrowheads point to the nuclear translocation of IL-33. Scale bar = 50 μm. G, Lung protein concentrations in lung tissue were determined via western blotting (n = 3), and (H) was the typical picture. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; and KO, knockout; WT, wild-type.
    Human Il 33 Concentration, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    IL-33 increased in the lung and BALF 1 h after LPS-induced lung injury. WT mice were administered an intratracheal injection of LPS, and then the lung tissue and BALF were collected for 1, 3, and 24 h after ARDS induction. A, The mRNA expression level of IL-33 was detected by quantitative polymerase chain reaction (n = 5). In the BALF (B) and lung (C), IL-33 protein concentrations were determined via ELISA (n = 5–8) and western blotting (E–D) (n = 5–8). Total protein in the lung (C) was measured by BCA. F, An anti-IL-33 antibody was used for the immunohistochemistry analysis of lung tissue (n = 5). IL-33 − / − mice were used as the negative controls. Arrowheads point to the nuclear translocation of IL-33. Scale bar = 50 μm. G, Lung protein concentrations in lung tissue were determined via western blotting (n = 3), and (H) was the typical picture. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; and KO, knockout; WT, wild-type.

    Journal: Shock (Augusta, Ga.)

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS

    doi: 10.1097/SHK.0000000000002114

    Figure Lengend Snippet: IL-33 increased in the lung and BALF 1 h after LPS-induced lung injury. WT mice were administered an intratracheal injection of LPS, and then the lung tissue and BALF were collected for 1, 3, and 24 h after ARDS induction. A, The mRNA expression level of IL-33 was detected by quantitative polymerase chain reaction (n = 5). In the BALF (B) and lung (C), IL-33 protein concentrations were determined via ELISA (n = 5–8) and western blotting (E–D) (n = 5–8). Total protein in the lung (C) was measured by BCA. F, An anti-IL-33 antibody was used for the immunohistochemistry analysis of lung tissue (n = 5). IL-33 − / − mice were used as the negative controls. Arrowheads point to the nuclear translocation of IL-33. Scale bar = 50 μm. G, Lung protein concentrations in lung tissue were determined via western blotting (n = 3), and (H) was the typical picture. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; and KO, knockout; WT, wild-type.

    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Techniques: Injection, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Translocation Assay, Knock-Out

    The IL-33/ST2 axis promotes lung damage in LPS-induced ARDS. BALF and lung tissue were harvested after LPS administration for 24 h. A, The lung D/W ratio was assessed to evaluate lung edema (n = 5). B, Protein concentration and neutrophil count (C) in the BALF were analyzed with a BCA kit and flow cytometry to detect epithelial permeability (n = 5). D and E, Histopathological images of the lung tissues are based on one representative image among five (HE staining), and the scale bar = 50 μm. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; ctr, control; D/W, dry/wet; HE, hematoxylin-eosin; KO, knockout; WT, wild-type.

    Journal: Shock (Augusta, Ga.)

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS

    doi: 10.1097/SHK.0000000000002114

    Figure Lengend Snippet: The IL-33/ST2 axis promotes lung damage in LPS-induced ARDS. BALF and lung tissue were harvested after LPS administration for 24 h. A, The lung D/W ratio was assessed to evaluate lung edema (n = 5). B, Protein concentration and neutrophil count (C) in the BALF were analyzed with a BCA kit and flow cytometry to detect epithelial permeability (n = 5). D and E, Histopathological images of the lung tissues are based on one representative image among five (HE staining), and the scale bar = 50 μm. The results are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; ctr, control; D/W, dry/wet; HE, hematoxylin-eosin; KO, knockout; WT, wild-type.

    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Techniques: Protein Concentration, Flow Cytometry, Permeability, Staining, Knock-Out

    IL-33 and ST2 deletion leads to reduced levels of circulating and BALF proinflammatory cytokines. The concentrations of cytokines in the serum and BALF were measured 24 h after ARDS induction and by CBA. The IL-6, MCP-1, and TNF levels in the plasma (A) and BALF (B) of WT mice, IL-33 − / − mice, and ST2 − / − mice were measured to evaluate the systemic inflammatory response (n = 5). The data are shown as the mean + SD * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; CBA, bead-based multiplex immunoassay; ctr, control; KO, knockout; WT, wild-type.

    Journal: Shock (Augusta, Ga.)

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS

    doi: 10.1097/SHK.0000000000002114

    Figure Lengend Snippet: IL-33 and ST2 deletion leads to reduced levels of circulating and BALF proinflammatory cytokines. The concentrations of cytokines in the serum and BALF were measured 24 h after ARDS induction and by CBA. The IL-6, MCP-1, and TNF levels in the plasma (A) and BALF (B) of WT mice, IL-33 − / − mice, and ST2 − / − mice were measured to evaluate the systemic inflammatory response (n = 5). The data are shown as the mean + SD * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; CBA, bead-based multiplex immunoassay; ctr, control; KO, knockout; WT, wild-type.

    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Techniques: Multiplex Assay, Knock-Out

    Mice lacking IL-33 and ST showed reduced ability to recruit and activate iNKT cells after ARDS induction. WT, IL-33 − / − , and ST2 − / − mice were subjected to a sham treatment or intratracheal LPS injection. After 24 h, the lung and spleen were obtained to prepare single-cell suspensions, and the cells were stained with anti-CD45, anti-CD3, anti-CD1d-tetramer, anti-NK1.1, anti-CD4, anti-CD8, and anti-CD69 antibodies. A and B, Representative dot plots. iNKT cells were identified as CD45 + CD3 + CD1d + tetramers (n = 5). C, The data are presented as the ratio of iNKT to CD3 + T cells and the CD69 geometric mean expression on iNKT cells in the lung and spleen (n = 3–5). D and E, Representative dot plots. NK cells were identified as CD45 + CD3 − NK1.1 + cells. F, The data are presented as the ratio of NK cells to CD3 − T cells and CD69 geometric mean expression on NK cells in the lung and spleen (n = 3–5). Three independent experiments were conducted to obtain the results shown. The data are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; Ctr, control; KO, knockout; WT, wild-type.

    Journal: Shock (Augusta, Ga.)

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS

    doi: 10.1097/SHK.0000000000002114

    Figure Lengend Snippet: Mice lacking IL-33 and ST showed reduced ability to recruit and activate iNKT cells after ARDS induction. WT, IL-33 − / − , and ST2 − / − mice were subjected to a sham treatment or intratracheal LPS injection. After 24 h, the lung and spleen were obtained to prepare single-cell suspensions, and the cells were stained with anti-CD45, anti-CD3, anti-CD1d-tetramer, anti-NK1.1, anti-CD4, anti-CD8, and anti-CD69 antibodies. A and B, Representative dot plots. iNKT cells were identified as CD45 + CD3 + CD1d + tetramers (n = 5). C, The data are presented as the ratio of iNKT to CD3 + T cells and the CD69 geometric mean expression on iNKT cells in the lung and spleen (n = 3–5). D and E, Representative dot plots. NK cells were identified as CD45 + CD3 − NK1.1 + cells. F, The data are presented as the ratio of NK cells to CD3 − T cells and CD69 geometric mean expression on NK cells in the lung and spleen (n = 3–5). Three independent experiments were conducted to obtain the results shown. The data are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; Ctr, control; KO, knockout; WT, wild-type.

    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Techniques: Injection, Staining, Expressing, Knock-Out

    The ratio of CD4 + T cells and CD8 + T cells to CD3 + T cells was not different after ARDS induction in IL-33 − / − and ST − / − mice. The lung and spleen were obtained to prepare single-cell suspensions, and the cells stained with anti-CD45, anti-CD3, anti-CD1d-tetramer, anti-NK1.1, anti-CD4, anti-CD8, and anti-CD69 antibodies 24 h after LPS-induced ARDS. A, The data are presented as the ratio of CD4 + T cells to CD3 + T cells and the CD69 geometric mean in CD4 + T cells in the lung and spleen (n = 5). B, The data are presented as the ratio of CD8+ T cells to CD3 + T cells and the CD69 geometric mean expression on CD8 + T cells in the lung and spleen (n = 5). The results shown are based on one of three independent experiments. The data are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; ctr, control; KO, knockout; WT, wild-type.

    Journal: Shock (Augusta, Ga.)

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS

    doi: 10.1097/SHK.0000000000002114

    Figure Lengend Snippet: The ratio of CD4 + T cells and CD8 + T cells to CD3 + T cells was not different after ARDS induction in IL-33 − / − and ST − / − mice. The lung and spleen were obtained to prepare single-cell suspensions, and the cells stained with anti-CD45, anti-CD3, anti-CD1d-tetramer, anti-NK1.1, anti-CD4, anti-CD8, and anti-CD69 antibodies 24 h after LPS-induced ARDS. A, The data are presented as the ratio of CD4 + T cells to CD3 + T cells and the CD69 geometric mean in CD4 + T cells in the lung and spleen (n = 5). B, The data are presented as the ratio of CD8+ T cells to CD3 + T cells and the CD69 geometric mean expression on CD8 + T cells in the lung and spleen (n = 5). The results shown are based on one of three independent experiments. The data are shown as the mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and n. s., not significant. ARDS, acute respiratory distress syndrome; ctr, control; KO, knockout; WT, wild-type.

    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Techniques: Staining, Expressing, Knock-Out

    IL-33 contributes to an uncontrolled inflammatory response in ARDS via activation of NKT cells. WT and Vα14Τg mice were pretreated with an anti-ST2 antibody 1 h before LPS administration and sacrificed 24 h after ARDS induction. BALF and lung tissue were harvested. A, The D/W ratio was calculated to evaluate lung edema (n = 5). B, Protein concentration and neutrophil count (C) in the BALF were measured with a BCA kit and by flow cytometry to evaluate epithelial permeability (n = 5). D and E, One of five representative histopathological images showing the lung tissues (HE staining). The lung injury score was determined by two blinded pathologists. Scale bar = 50 μm. F, The levels of IL-6, MCP-1, and TNF in the plasma and BALF were tested by CBA. (n = 5, * P < 0.05). The data are shown as the mean ± SD and are representative of three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; ctr, control; D/W, dry/wet; KO, knockout; WT, wild-type.

    Journal: Shock (Augusta, Ga.)

    Article Title: THE IL-33/ST2 AXIS PROMOTES ACUTE RESPIRATORY DISTRESS SYNDROME BY NATURAL KILLER T CELLS

    doi: 10.1097/SHK.0000000000002114

    Figure Lengend Snippet: IL-33 contributes to an uncontrolled inflammatory response in ARDS via activation of NKT cells. WT and Vα14Τg mice were pretreated with an anti-ST2 antibody 1 h before LPS administration and sacrificed 24 h after ARDS induction. BALF and lung tissue were harvested. A, The D/W ratio was calculated to evaluate lung edema (n = 5). B, Protein concentration and neutrophil count (C) in the BALF were measured with a BCA kit and by flow cytometry to evaluate epithelial permeability (n = 5). D and E, One of five representative histopathological images showing the lung tissues (HE staining). The lung injury score was determined by two blinded pathologists. Scale bar = 50 μm. F, The levels of IL-6, MCP-1, and TNF in the plasma and BALF were tested by CBA. (n = 5, * P < 0.05). The data are shown as the mean ± SD and are representative of three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid; ctr, control; D/W, dry/wet; KO, knockout; WT, wild-type.

    Article Snippet: According to the manufacturer’s instructions, IL-33 concentrations in mouse serum and lung tissue were tested using ELISA kits (R&D Systems, Abingdon, United Kingdom).

    Techniques: Activation Assay, Protein Concentration, Flow Cytometry, Permeability, Staining, Knock-Out